【Scope of application】
None
【Relevant Standards】
【Technical Parameters】
1. Sample capacity: 96-well module, which can use 0.2ml single tube, octa-tube, etc.;
2. Recommended optimal reaction system: 10-100ul;
3. Temperature control accuracy of the reaction module: ±0.25°C;
4. Temperature control mode: the heating method adopts Peltier effect system or semiconductor; Cooling technology uses semiconductors;
5. Standard high-speed reaction module, maximum temperature change rate: 5°C/s;
6. Temperature control uniformity of reaction module: ±0.4°C;
7. With temperature gradient function; It can optimize 6 temperature points at the same time, which can be used to quickly optimize reaction conditions;
8. Optical system: standard 4 detection channel modules, to meet the simultaneous 4-color fluorescence detection, the instrument reserves the detection channel upgrade bit, to meet the upgrade of up to 6 channels of detection, or 6 detection channel modules, to meet the simultaneous 6-color fluorescence detection;
9. Detector: high-sensitivity photomultiplier tube (PMT), photodiode (PN/PIN) or low-temperature high-resolution CMOS;
10. Light source: 6 excitation channels;
11. Excitation spectral range: 450-730nm;
12. Detection linear range: 10 orders of magnitude;
13. Detection sensitivity: can detect single-copy DNA templates;
14. Multiple data analysis: standard software can analyze fluorescence data of no less than 4 detection channels at the same time;
15. Data analysis mode: data analysis functions such as standard curve quantification, melting curve, ΔCT or ΔΔCT gene expression analysis, allele analysis, and amplification efficiency-based data analysis mode;
16. Supported fluorescent dyes: FAM/SYBR, VIC/TET/HEX, ROX/ Texas Red, Cy5, fluorescent dyes with similar spectra;
17. Display: The host comes with an 8.5-inch large screen color touch screen, a touchpad and can view real-time PCR experiments;
18. Support high-resolution melting point curve analysis function (HRM), melting point curve resolution 0.1°C;
19. Data export: Excel, Word, or PowerPoint. User reports contain run settings, graphical and tabular data results that can be printed or saved directly;
20. It has control and analysis software in Chinese and English, which can be switched freely, and the interface is simple and intuitive.
21. The consumables and kits of the instrument are open; Users can choose a variety of consumables and reagents from the same brand as the original manufacturer, as well as consumables and reagents from other manufacturers on the market.