【Scope of application】   【Relevant Standards】

This equipment fully meets the testing requirements of the test method in Appendix B of YY 0469-2011.

[Instrument features]: 1. This equipment fully meets the testing requirements of Appendix B test method in YY 0469-2011. 2. The instrument is simple to operate, easy to connect, and only one button operation can be completed after connecting the test piece, improving the testing efficiency. 3. The negative pressure test system of the biological safety cabinet is adopted, with fan exhaust system and inlet and outlet air filters to ensure the safety of operators. The biological safety cabinet is equipped with a lifting glass door for easy observation. 4. The operating system adopts industrial-grade PLC, which has accurate data processing and can support flexible and free parameter setting and experimental data storage. 5. The control terminal adopts high-brightness color Chinese touch screen operation, the operation interface is friendly, simple and easy to understand. 6. All core components are selected from well-known brand original imported components, with higher detection accuracy and repeatability. 7. The equipment has a good protection function, which automatically starts the protection function when the detection data exceeds the limit, which prevents damage to the equipment caused by misoperation and reduces the difficulty of operation by inspectors. 8. The equipment is equipped with microcomputer to control the automatic start-stop pressure source, which is simple to operate, and the pressure comes from the pressure buffer tank, and the output pressure is more stable. 【Technical parameters】： 1. Positive pressure source and air filtration system, positive pressure control output 5~500kPa adjustable; 2. Negative pressure source and filtration system, provide negative pressure air source not less than 60L/MIN; 3. Special aerosol generator to provide aerosols with particle size and concentration that meet the experimental requirements; 4. Precision flow control: 0~30L range; 5. Equipped with a special sampler to realize the clamping of the tested piece; 6. LCD touch screen display and operation; 7. PLC control; Power supply: 220V/50Hz; Test time: 0-3600S can be set. 【Air schematic diagram】： 1. The test device mainly consists of an aerosol generator, an aerosol chamber, a sampler and a vacuum air source. 1.1 The core components of the aerosol generator are the aerosol generator imported from the UK and the pressure control unit imported from Japan, which controls the gas pressure applied to the aerosol generator to produce the appropriate concentration and particle size of the aerosol, and the gas pressure is controlled to 0.1kPa, which can be adjusted in real time during the experiment. 1.2 The dosing device of the aerosol generator considers peristaltic pump administration to be intermittent at a flow rate of 0.01ML/MIN. Therefore, if you choose an aerosol generator with a reservoir, you can directly fill the pharmaceutical industry into the generator and adjust it by adjusting the spray nozzle. 1.3 The positive pressure air source is provided by an oil-free air compressor, filtered by the air filter and connected to the pressure control unit, and the pressure control is adjustable by 5kPa~500kPa. 2. The aerosol chamber is made with reference to Figure B.2, with two sampler interfaces and reserved filter interfaces. 3. The sampler is a two-way Anderson sampler, and the rear end of the sampler is controlled by a flow switch with adjustable flow, and the flow control range is adjustable from 0~50L. Gas drying is required between the sampler and the flow meter. 4. The vacuum air source is provided by two vacuum pumps, each of which is capable of providing a negative pressure gas flow rate of not less than 30L/MIN. 【Program functions】： 1. Open settings for each experimental step: Configure the parameters of all the steps in the experiment according to a unified timeline, such as generator working time, sampler working time, etc., and all open on and off times. The experimental process can be freely matched, making it more flexible and intuitive. 2. Negative pressure inlet pressure protection function, real-time monitoring of positive pressure inlet pressure to ensure that the gas demand of the aerosol generator can be met during the experiment process and ensure the production of qualified aerosols. Otherwise, the alarm will be called and the experiment will be stopped. 3. Negative pressure source protection function, real-time monitoring of negative pressure pressure and flow during the experiment to ensure that the experimental conditions meet the standard requirements, otherwise the alarm will be alarmed and the experiment will be stopped. 4. Positive pressure gas automatic control function, only need to input the pressure required by the generator to work, the internal algorithm of the system will automatically adjust the feedback pressure, which can quickly stabilize at the required pressure. 【Experimental process】： 1. Open the power supply and valve of the air compressor vacuum pump and inflate it. 2. Connect all parts of the pipeline. 3. Connect the Anderson sampler. 4. Add the experimental bacterial solution to the sprayer. 6. Set the test parameters according to the experimental time in standard B.4 on the parameter setting interface. 7. Follow the experimental steps in B.4 to conduct positive quality control, sample challenge and negative quality control experiments in turn. 8. Culture the agar plate. 9. Clean the inside of the equipment and pipelines for the next test.